Addgene - pML1335 Plasmid Data

Plasmid 32377: pML1335

Gene/insert name:
int L5; gfpm2+; xylEm; ttsbiA, ttsbiB
Vector backbone:
pML1342
(Search Vector Database)
Vector type:
bacterial integration vector
Cloning site 5':
Pme I
Site destroyed during cloning:
No
Cloning site 3':
Cla I
Site destroyed during cloning:
No
5' sequencing primer:
psmyc1 List of Sequencing Primers
Bacterial resistance(s)
Hygromycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Michael Niederweis
Terms and Licenses:
MTA

Comments:

This is an improved L5 integration vector for stable gene integration in mycobacteria with codon optimized gfp and xylE as reporter genes; terminators to protect expression cassette against transcriptional
interference.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Taking phage integration to the next level as a genetic tool for mycobacteria. Huff et al (Gene. 2010 Nov 15;468(1-2):8-19. Epub 2010 Aug 6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32377" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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