Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32387||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4818
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namep107 promoter
SpeciesM. musculus (mouse)
Insert Size (bp)900
Mutation1st and 2nd E2F binding sites mutated: GCG-->AAA and GTC-->AAA
Entrez GeneRbl1 (a.k.a. AW547426, PRB1, p10, p107)
/ Fusion Protein
- Luciferase (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site None (unknown if destroyed)
- 3′ cloning site None (unknown if destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer Luc_N_Rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL3-p107 1*2* was a gift from Julien Sage (Addgene plasmid # 32387 ; http://n2t.net/addgene:32387 ; RRID:Addgene_32387)
For your References section:Tandem E2F binding sites in the promoter of the p107 cell cycle regulator control p107 expression and its cellular functions. Burkhart DL, Wirt SE, Zmoos AF, Kareta MS, Sage J. PLoS Genet. 2010 Jun 24;6(6):e1001003. 10.1371/journal.pgen.1001003 PubMed 20585628