Addgene - pAAV-GFP Plasmid Data

Plasmid 32395: pAAV-GFP

Gene/insert name:
GFP
Vector backbone:
pSub201
(Search Vector Database)
Vector type:
Mammalian Expression ; Adeno-Associated Virus
Backbone size w/o insert (bp):
8310
Promoter:
CMV
Cloning site 5':
Multiple (see map)
Site destroyed during cloning:
No
Cloning site 3':
Multiple (see map)
Site destroyed during cloning:
No
5' sequencing primer:
GGCCCTTTTGCTAATCATGTTCATACCTCTTATCT List of Sequencing Primers
3' sequencing primer:
GGCCAGGGCATTAGCCACACCAG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl2
Growth temperature (℃):
37
Growth instructions:
Users must confirm the absence of deletions (recombinations) in this vector by diagnostic digests. (1) A SmaI digest should produce the following pattern of bands: 3654 bp, 1716 bp and 1500 bp. (2) An AhdI digest should produce the following pattern of bands: 3183 bp, 2582 bp and 1127 bp. Please consult the associated publication listed below for an example gel image.
High or low copy:
Low Copy
Sequence: View sequences (2)
Map:
View map
Supplemental document:
Notes from Addgene (2)
Principal Investigator:
John_T Gray
Terms and Licenses:
MTA

Comments:

Please see Notes from Addgene link above for Addgene's diagnostic digests of this plasmid with AhdI and SmaI.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Design and Construction of Functional AAV Vectors. Gray et al (Methods Mol Biol. 2011;807:25-46. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32395" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

32396: pscAAV-GFP

16399: AdEasier-1 cells (strain)

20342: FUW-M2rtTA

16400: pAdEasy-1

16404: pAdTrack