LHP1463 - INP52
(Plasmid #32432)

• Depositing Lab: Linda Hicke
• Publication: Stamenova et al J Biol Chem. 2004 Apr 16;279(16):16017-25. Epub 2004 Feb 3.

Backbone

• Vector backbone: YCplac22
• Backbone manufacturer: ATCC
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: INP52
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: INP52 (a.k.a. YNL106C, SJL2)
• Promoter: Lac

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PstI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: M13 reverse
• 3′ sequencing primer: M13 forward

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

INP52 was amplified from yeast genomic DNA using 5′ PstI and 3′ BamHI site-containing oligonucleotides, digested and ligated into PstI- and BamHI-digested YCplac22. The NotI site was inserted at the 5′-end of INP52 via QuikChange™ mutagenesis. A triple HA epitope was ligated into the NotI site to generate pHA-INP52 (LHP1463).