Addgene - LHP1035 - ECFP-Cterm Plasmid Data

Plasmid 32435: LHP1035 - ECFP-Cterm

Gene/insert name:
ECFP
Vector backbone:
pCR2.1 TOPO
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Cloning
Cloning method:
TOPO Cloning
5' sequencing primer:
M13 Reverse List of Sequencing Primers
3' sequencing primer:
M13 Forward
Bacterial resistance(s)
Ampicillin and Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Linda Hicke
Terms and Licenses:
MTA

Comments:

For the addition of ECFP to the C-terminus of another cDNA by cloning the cDNA into the BamHI site (of the original pCR2.1 TOPO).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32435" in your Materials and Methods section.