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pEGFPC1-GluR1Ctail
(Plasmid #32441)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32441 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GluR1
  • Species
    H. sapiens (human)
  • Mutation
    Contains the last C-terminal 81 amino acids of the GluR1 subunit
  • Entrez Gene
    GRIA1 (a.k.a. GLUH1, GLUR1, GLURA, GluA1, HBGR1)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40par
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFPC1-GluR1Ctail was a gift from Robert Malinow (Addgene plasmid # 32441 ; http://n2t.net/addgene:32441 ; RRID:Addgene_32441)
  • For your References section:

    Postsynaptic receptor trafficking underlying a form of associative learning. Rumpel S, LeDoux J, Zador A, Malinow R. Science. 2005 Apr 1;308(5718):83-8. Epub 2005 Mar 3. 10.1126/science.1103944 PubMed 15746389