Addgene - pHAGE-CMV-Rheb(D60I)-IRES-eGFP-W Plasmid Data

Plasmid 32521: pHAGE-CMV-Rheb(D60I)-IRES-eGFP-W

Gene/insert name:
Dominant negative Rheb (D60I)
Species:
R. norvegicus (rat)
Entrez Gene:
Rheb ()
Mutation:
D60I to make dominant negative
Vector backbone:
pHAGE-CMV-Rheb-IRES-eGFP-W
(Search Vector Database)
Backbone manufacturer:
Addgene plasmid #32519
Vector type:
Mammalian Expression, Lentiviral
Promoter:
CMV
Cloning site 5':
NotI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
CMV forward List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Mustafa Sahin
Terms and Licenses:
MTA

Comments:

The published dominant negative D60I mutation was introduced to pHAGE-CMV-Rheb-IRES-eGFP-W by site directed mutagenesis. Although reported to have robust DN activity in other cell types, this construct did not provide robust DN activity in neurons.

The original pHAGE-CMV-IRES lentiviral vector is from Dr Richard C. Mulligan at Harvard (Mostoslavsky G et al., 2005).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32521" in your Materials and Methods section.