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pBABe-HA-ER-IPpoI
(Plasmid #32565)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32565 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBABE-Puro
  • Backbone manufacturer
    margenstern and L (1991) PMID Z194165
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    I-PpoI
  • Species
    Physarum polycephalum
  • Insert Size (bp)
    1514
  • Mutation
    none
  • GenBank ID
    PMD 17473858
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • ER (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer PBABE 5'
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABe-HA-ER-IPpoI was a gift from Michael Kastan (Addgene plasmid # 32565 ; http://n2t.net/addgene:32565 ; RRID:Addgene_32565)
  • For your References section:

    Roles of ATM and NBS1 in chromatin structure modulation and DNA double-strand break repair. Berkovich E, Monnat RJ Jr, Kastan MB. Nat Cell Biol. 2007 Jun;9(6):683-90. Epub 2007 May 7. 10.1038/ncb1599 PubMed 17486112