|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32585||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepDONR221 P3-P2
Vector typeGateway cloning vector
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameIntronic HCN1miRNA and EGFP
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 Forward (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe HCN1miR was cloned into pSM155-GFP provided by Guangwei Du, University of Texas Health Science Center, Houston. A cassette containing the intronic miRNA upstream of EGFP was then amplified with PCR primers containing att sites and recombined to generate the entry vector.
Terms and Licenses
HCN1miRNA sequence TAAAGACGACAGTAGGTATCAGT
Du, G., Yonekubo, J., Zeng, Y., Osisami, M., and Frohman, M. A. (2006). Design of expression vectors for RNA interference based on miRNAs and RNA splicing. FEBS J. 273, 5421–5427.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pENTR-L3-HCN1miR-EGFP-L2 was a gift from Matthew Nolan (Addgene plasmid # 32585)
For your References section:A Molecular Toolbox for Rapid Generation of Viral Vectors to Up- or Down-Regulate Neuronal Gene Expression in vivo. White MD, Milne RV, Nolan MF. Front Mol Neurosci. 2011;4:8. Epub 2011 Jul 4. 10.3389/fnmol.2011.00008 PubMed 21772812
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.