|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32615||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerMark Emerson
- Backbone size w/o insert (bp) 6610
Vector typeMammalian Expression
Growth in Bacteria
SpeciesG. gallus (chicken)
Insert Size (bp)54
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer GTTCCGCGCACATTTCCCCG (Common Sequencing Primers)
Terms and Licenses
Note that there are some minor discrepancies between Addgene's quality control sequence and the depositor's assembled sequence. These differences should not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:2XDR4 was a gift from Connie Cepko (Addgene plasmid # 32615 ; http://n2t.net/addgene:32615 ; RRID:Addgene_32615)
For your References section:Analysis of thyroid response element activity during retinal development. Billings NA, Emerson MM, Cepko CL. PLoS One. 2010 . 5(10):e13739. 10.1371/journal.pone.0013739 PubMed 21060789