Addgene - pSADdeltaG-GFP-rtTA Plasmid Data

Plasmid 32648: pSADdeltaG-GFP-rtTA

Gene/Insert 1
Gene/insert name:
GFP
Insert size:
700
Gene/Insert 2
Gene/insert name:
rtTA
Insert size:
800
Vector backbone:
pSADdeltaG
(Search Vector Database)
Backbone manufacturer:
Addgene plasmid 32634
Vector type:
Mammalian Expression ; Rabies virus
Backbone size w/o insert (bp):
15428
Cloning information for Gene/Insert 1
Promoter:
CMV
Cloning site 5':
NA
Site destroyed during cloning:
Unknown
Cloning site 3':
NA
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
BGH-rev
Cloning information for Gene/Insert 2
Cloning site 5':
NA
Site destroyed during cloning:
Unknown
Cloning site 3':
NA
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
BGH-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (5)
Map:
View map
Principal Investigator:
Edward Callaway
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: New rabies virus variants for monitoring and manipulating activity and gene expression in defined neural circuits. Osakada et al (Neuron. 2011 Aug 25;71(4):617-31. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32648" in your Materials and Methods section.