Addgene - pRK5-GRK6 Plasmid Data

Plasmid 32693: pRK5-GRK6

Gene/insert name:
GRK6
Species:
H. sapiens (human)
Entrez Gene:
GRK6 (GPRK6)
Vector backbone:
pRK5
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4754
Promoter:
CMV
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-Fwd List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Robert Lefkowitz
Terms and Licenses:
MTA

Comments:

A-variant of GRK6. The insert includes the 3'UTR.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Phosphatidylinositol 4,5-bisphosphate (PIP2)-enhanced G protein-coupled receptor kinase (GRK) activity. Location, structure, and regulation of the PIP2 binding site distinguishes the GRK subfamilies. Pitcher et al (J Biol Chem. 1996 Oct 4. 271(40):24907-13. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32693" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

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25434: pMA2640