Addgene - pCol-TGM-luc.1309 Plasmid Data

Plasmid 32720: pCol-TGM-luc.1309

Gene/insert name:
luciferase
shRNA sequence:
ctcgagaaggtatattgctgttgacagtgagcgcccgcctgaagtctctgattaatagt gaagccacagatgtattaatcagagacttcaggcggttgcctactgcctcggaattc
Vector backbone:
pBS31'-RBGpA
(Search Vector Database)
Backbone manufacturer:
Jaensich laboratory
Vector type:
Mouse Targeting, RNAi
Backbone size w/o insert (bp):
6120
Modifications to Backbone:
GFP-miR30 cassette addition Xho mutation
Promoter:
TRE
Promoter:
Tre
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
EGFP-C List of Sequencing Primers
3' sequencing primer:
Bglob-intron-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Top10/P3
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Principal Investigator:
Scott Lowe
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A rapid and scalable system for studying gene function in mice using conditional RNA interference. Premsrirut et al (Cell. 2011 Apr 1;145(1):145-58. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32720" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with

20730: Col1a-frt-hygro-pA