Gene/insert name: MLH1
Alt name: PDB: 3RBN
Alt name: MLH1 mutL homolog 1, DNA-mismatch domain
Insert size: 798
Species:
H. sapiens (human)
Entrez Gene:
MLH1
(COCA2, FCC2, HNPCC, HNPCC2, hMLH1)
Fusion protein or tag: His (with TEV cleavage)
Terminal: N terminal on backbone
Mutation: contains amino acids 486-751
Vector backbone:
pET28-MHLSearch Vector Database )
Backbone manufacturer:
Structural Genomics Consortium (Addgene plasmid 26096)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
7325
Cloning method: Ligation Independent Cloning
5' sequencing primer:
T7
List of Sequencing Primers
3' sequencing primer:
T7-term
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence:
View sequences (3)
Principal Investigator:
Cheryl Arrowsmith
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter
Addgene has sequenced a portion of this plasmid for verification.
Click here for the sequencing
result.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
32859" in your Materials and Methods section.
PDB: 3RBN. http://www.thesgc.org/structures/details?pdbid=...