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p4973 pcDNA4-E6AP III C21Y
(Plasmid #32890)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 32890 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    E6AP
  • Species
    H. sapiens (human)
  • Mutation
    C21Y
  • Entrez Gene
    UBE3A (a.k.a. ANCR, AS, E6-AP, EPVE6AP, HPVE6A)
  • Promoter CMV
  • Tags / Fusion Proteins
    • 6 His Express (N terminal on backbone)
    • T7-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoR V (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p4973 pcDNA4-E6AP III C21Y was a gift from Peter Howley (Addgene plasmid # 32890)
  • For your References section:

    Biochemical analysis of Angelman syndrome-associated mutations in the E3 ubiquitin ligase E6-associated protein. Cooper EM, Hudson AW, Amos J, Wagstaff J, Howley PM. J Biol Chem. 2004 Sep 24;279(39):41208-17. Epub 2004 Jul 19. 10.1074/jbc.M401302200 PubMed 15263005