Addgene - Mef2D-shRNA Plasmid Data

Plasmid 32900: Mef2D-shRNA

Gene/insert name:
Mef2d
shRNA sequence:
5’- GTAGCTCTCTGGTCACTCC -3’
Species:
R. norvegicus (rat)
Entrez Gene:
Mef2d ()
Vector backbone:
pSuper
(Search Vector Database)
Backbone manufacturer:
OligoEngine
Vector type:
Mammalian Expression, RNAi
Backbone size w/o insert (bp):
3176
Promoter:
H1-RNA
Cloning site 5':
none
Site destroyed during cloning:
Unknown
Cloning site 3':
none
Site destroyed during cloning:
Unknown
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
M13 reverse
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Michael Greenberg
Terms and Licenses:
MTA

Comments:

Can be used in conjunction with pcDNA3-rMEF2D (Addgene #32902) which is mutated so as to not be targeted by this shRNA.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Activity-dependent regulation of MEF2 transcription factors suppresses excitatory synapse number. Flavell et al (Science. 2006 Feb 17;311(5763):1008-12. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32900" in your Materials and Methods section.