Modified Shipping Schedule: Addgene will be closed November 23rd & 24th for the Thanksgiving holiday. Order processing and shipping may be delayed the week of Nov 20 - 24. If you have any questions, please contact us at [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pEGFP-rNFM
(Plasmid #32907)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32907 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4134
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP-rat NFM fusion
  • Alt name
    NFM
  • Alt name
    neurofilament protein M
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    3330
  • Entrez Gene
    Nefm (a.k.a. Nef3, Nfm)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ApaI (not destroyed)
  • 3′ cloning site ApaI (destroyed during cloning)
  • 5′ sequencing primer multiple primers
  • 3′ sequencing primer multiple primers
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Plasmid first described in Wang et al. (2000) "Rapid movement of axonal neurofilaments interrupted by prolonged pauses" Nature Cell Biology 2:137-141.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-rNFM was a gift from Anthony Brown (Addgene plasmid # 32907)
  • For your References section:

    Rapid movement of axonal neurofilaments interrupted by prolonged pauses. Wang L, Ho CL, Sun D, Liem RK, Brown A. Nat Cell Biol. 2000 Mar;2(3):137-41. 10.1038/35004008 PubMed 10707083