|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32994||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3200
Growth in Bacteria
Gene/Insert nameactivin B
SpeciesX. laevis (frog)
Insert Size (bp)1200
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (destroyed during cloning)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
Linearize with EcoRI and transcribe with SP6 for sense XActivin bB mRNA.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:XActB-pSP64T was a gift from Sergei Sokol (Addgene plasmid # 32994 ; http://n2t.net/addgene:32994 ; RRID:Addgene_32994)
For your References section:Injected Wnt RNA induces a complete body axis in Xenopus embryos. Sokol S, Christian JL, Moon RT, Melton DA. Cell. 1991 Nov 15;67(4):741-52. 10.1016/0092-8674(91)90069-B PubMed 1834344
Map uploaded by the depositor.