Addgene - pCMV-Flag-YAP-S109A Plasmid Data

Plasmid 33088: pCMV-Flag-YAP-S109A

Gene/insert name:
YAP
Entrez Gene:
YAP1 (YAP, YAP2, YAP65, YKI)
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Mutation:
S109A
Vector backbone:
pCMV
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5000
Promoter:
CMV
Cloning site 5':
NA
Site destroyed during cloning:
Unknown
Cloning site 3':
NA
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
hGH-pA-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Kunliang Guan
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Inactivation of YAP oncoprotein by the Hippo pathway is involved in cell contact inhibition and tissue growth control. Zhao et al (Genes Dev. 2007 Nov 1. 21(21):2747-61. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 33088" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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