pRS314-T7-GFP
(Plasmid #33130)

• Depositing Lab: Michael Rosbash
• Publication: Dower et al RNA. 2002 May;8(5):686-97.

Backbone

• Vector backbone: pRS314
• Backbone manufacturer: ATCC
• Backbone size w/o insert (bp): 4783
• Modifications to backbone: NotI - T7 promoter - TDH3 5'UTR - BamHI - GFP - SalI - TDH3 3' UTR and polyadenylation signal - EcoRI
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): TG1
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP with TDH3 5' and 3' UTR
• Entrez Gene: gfp (a.k.a. pCmGFP_001)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: T7 forward

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

T7 promoter driven GFP with the TDH3 5' and 3' flanking sequences, and the TDH3 polyadenylation signal all cloned into pRS314 using NotI and EcoRI.