Addgene - pLG-Nde-Acc Plasmid Data

Plasmid 33150: pLG-Nde-Acc

Gene/insert name:
RP51A-synthetic minimal intron
Species:
S. cerevisiae (budding yeast)
Entrez Gene:
RPS17A (YML024W, RP51A, RPL51A)
Fusion protein or tag:
LacZ
Terminal:
C terminal on backbone
Mutation:
AccI site in intron was cut/filled/ligated to change GTCGAC to GTCGCGAC, and NdeI site after intron was cut/filled and a BamHI linker was inserted to obtain the appropriate reading frame changing CATATG to CATACGGGATCC
Vector backbone:
pLGSD5
(Search Vector Database)
Vector type:
Yeast Expression
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
none List of Sequencing Primers
3' sequencing primer:
LacZ-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
TG1
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
URA3
Sequence: View sequences (1)
Map:
View map
Map:
View map
Principal Investigator:
Michael Rosbash
Terms and Licenses:
MTA

Comments:

Original intron sequence: GTATGTTAATATGGTTAACGTCGACCGTGTTTTTGATATCTATACTAACAGGCCTTTTAATAG

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm. Legrain et al (Cell. 1989 May 19;57(4):573-83. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 33150" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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