pAC-Luc
(Plasmid #33154)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pAc5.1/V5-His B
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 5400
  • Modifications to backbone
    A PCR product containing the Firefly Luciferase gene and an artificial poly-linker sequence was ligated into pAC5.1/V5-His B between the 5' KpnI and 3' XhoI sites. Primers: Forward: GGATCGGGGTACCTATGGAAGACGCCAAAAACATAAAG Reverse: CACTAGACTCGAGCGTTACAATTTGGACTTTCCGCC Because of the stop codon, V5 and His Tags are not expressed. Restriction sites 3' of Luciferase are Xho1-XbaI-ApaI-BtgI-SacII-V5-MluI-AgeI-6xHIS-PmeI-DraI-StuI-SacI.
  • Vector type
    Insect Expression, Retroviral, Luciferase
  • Promoter Actin 5C
  • Selectable markers
    Hygromycin, Blasticidin
  • Tag / Fusion Protein
    • Luciferase (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAC-Luc was a gift from Michael Rosbash (Addgene plasmid # 33154)
  • For your References section:

    Clockwork Orange is a transcriptional repressor and a new Drosophila circadian pacemaker component. Kadener S, Stoleru D, McDonald M, Nawathean P, Rosbash M. Genes Dev. 2007 Jul 1;21(13):1675-86. Epub 2007 Jun 19. 10.1101/gad.1552607 PubMed 17578907