Gal-Rad51 KA
(Plasmid
#33231)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 33231 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepYES2
- Backbone size w/o insert (bp) 5857
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRad51
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SpeciesS. cerevisiae (budding yeast)
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MutationK191A
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Entrez GeneRAD51 (a.k.a. YER095W, MUT5)
- Promoter Gal1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site SphI (unknown if destroyed)
- 5′ sequencing primer Gal1_primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Gal-Rad51 KA was a gift from Hannah Klein (Addgene plasmid # 33231 ; http://n2t.net/addgene:33231 ; RRID:Addgene_33231) -
For your References section:
Swi2/Snf2-related translocases prevent accumulation of toxic Rad51 complexes during mitotic growth. Shah PP, Zheng X, Epshtein A, Carey JN, Bishop DK, Klein HL. Mol Cell. 2010 Sep 24;39(6):862-72. 10.1016/j.molcel.2010.08.028 PubMed 20864034