Addgene - Gal-Rad51 KR Plasmid Data

Plasmid 33232: Gal-Rad51 KR

Gene/insert name:
Rad51
Species:
S. cerevisiae (budding yeast)
Entrez Gene:
RAD51 (YER095W, MUT5)
Mutation:
K191R
Vector backbone:
pYES2
(Search Vector Database)
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
5857
Promoter:
Gal1
Cloning site 5':
HindIII
Site destroyed during cloning:
Unknown
Cloning site 3':
SphI
Site destroyed during cloning:
Unknown
5' sequencing primer:
Gal1_primer List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
URA3
Sequence: View sequences (1)
Principal Investigator:
Hannah Klein
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Swi2/Snf2-related translocases prevent accumulation of toxic Rad51 complexes during mitotic growth. Shah et al (Mol Cell. 2010 Sep 24;39(6):862-72. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 33232" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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