pCasper_Pdf-LexA::VP16
(Plasmid #33259)

• Depositing Lab: Michael Rosbash
• Publication: Shang et al Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):19587-94. Epub 2008 Dec 5.

Backbone

• Vector backbone: pCasPeR3
• Modifications to backbone: The 2.4-kb Pdf promoter (Stoleru et al., 2004) was cloned into pCasPeR3 between BamHI and BglII sites.
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LexA::VP16-SV40 3'UTR
• Species: E. coli
• Entrez Gene: lexA (a.k.a. b4043, ECK4035, exrA, spr, tsl, umuA)
• Promoter: Pdf

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: SV40 polyA reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A XbaI site was added to the 3' end of LexAVP16-SV40 3'UTR by PCR from pBS-LexAVP16 plasmid (Lai and Lee, 2006).
The PCR fragment was then subcloned into the XbaI site of the pBS vector.
LexAVP16-SV40 was then released by NotI digestion and inserted into the NotI site in pCaspeR3, downstream of Pdf promoter.