Addgene - pT7-L1-S1T3D Plasmid Data

Plasmid 33299: pT7-L1-S1T3D

Gene/Insert 1
Gene/insert name:
L1
Species:
Orthoreovirus T3D
Entrez Gene:
L1 (MoV3sL1gp1)
L1 (MoV-3mpc04sL1_gp1)
Gene/Insert 2
Gene/insert name:
S1
Species:
Orthoreovirus T3D
Entrez Gene:
S1 (MoV-3mpc04sS1_gp1)
Vector backbone:
p3E5EGFP
(Search Vector Database)
Backbone manufacturer:
made by Yoshihiro Kawaoka
Vector type:
Mammalian Expression
Cloning information for Gene/Insert 1
Promoter:
T7
Cloning site 5':
none
Site destroyed during cloning:
Unknown
Cloning site 3':
none
Site destroyed during cloning:
Unknown
5' sequencing primer:
ovL1T3D-F List of Sequencing Primers
Cloning information for Gene/Insert 2
Promoter:
T7
Cloning site 5':
none
Site destroyed during cloning:
Unknown
Cloning site 3':
none
Site destroyed during cloning:
Unknown
5' sequencing primer:
none List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Terence Dermody
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: An improved reverse genetics system for mammalian orthoreoviruses. Kobayashi et al (Virology. 2010 Mar 15;398(2):194-200. Epub 2009 Dec 29. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 33299" in your Materials and Methods section.