Gene/Insert 1
Gene/insert name: S2
Species: Orthoreovirus T3D
Entrez Gene:
S2
(MoV-3mpc04sS2_gp1)
Gene/Insert 2
Gene/insert name: S3
Species: Orthoreovirus T3D
Entrez Gene:
S3
(MoV-3mpc04sS3_gp1)
Gene/Insert 3
Gene/insert name: S4
Species: Orthoreovirus T3D
Entrez Gene:
S4
(MoV-3mpc04sS4_gp1)
Gene/Insert 4
Gene/insert name: M2
Species: Orthoreovirus T3D
Entrez Gene:
M2
(MoV-3mpc04sM2_gp1)
Vector backbone:
p3E5EGFP
(Search Vector Database )
Backbone manufacturer:
made by Yoshihiro Kawaoka
Vector type:
Mammalian Expression
Cloning information for Gene/Insert 1
Promoter: T7
Cloning site 5':
none
Site destroyed during cloning:
Unknown
Cloning site 3':
none
Site destroyed during cloning:
Unknown
5' sequencing primer:
none
List of Sequencing Primers
3' sequencing primer:
ovS2T3D-F
Cloning information for Gene/Insert 2
Promoter: T7
Cloning site 5':
none
Site destroyed during cloning:
Unknown
Cloning site 3':
none
Site destroyed during cloning:
Unknown
5' sequencing primer:
none
List of Sequencing Primers
Cloning information for Gene/Insert 3
Promoter: T7
Cloning site 5':
none
Site destroyed during cloning:
Unknown
Cloning site 3':
none
Site destroyed during cloning:
Unknown
5' sequencing primer:
none
List of Sequencing Primers
Cloning information for Gene/Insert 4
Promoter: T7
Cloning site 5':
none
Site destroyed during cloning:
Unknown
Cloning site 3':
none
Site destroyed during cloning:
Unknown
5' sequencing primer:
ovM2T3D-R
List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence:
View sequences (5)
Principal Investigator:
Terence Dermody
Terms and Licenses:
MTA
Addgene has sequenced a portion of this plasmid for verification.
Click here for the sequencing
result.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
33302" in your Materials and Methods section.