Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at help@addgene.org. Learn more

pT7-M2-S2-S3-S4T3D
(Plasmid #33302)

Add to Cart
Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    p3E5EGFP
  • Backbone manufacturer
    made by Yoshihiro Kawaoka
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Sequence Information

Gene/Insert 1

  • Gene/Insert name
    S2
  • Species
    Orthoreovirus T3D
  • Entrez Gene
    S2 (a.k.a. MoV-3mpc04sS2_gp1)
  • Promoter T7

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer ovS2T3D-F
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    S3
  • Species
    Orthoreovirus T3D
  • Entrez Gene
    S3 (a.k.a. MoV-3mpc04sS3_gp1)
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer none
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    S4
  • Species
    Orthoreovirus T3D
  • Entrez Gene
    S4 (a.k.a. MoV-3mpc04sS4_gp1)
  • Promoter T7

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer none
  • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    M2
  • Species
    Orthoreovirus T3D
  • Entrez Gene
    M2 (a.k.a. MoV-3mpc04sM2_gp1)
  • Promoter T7

Cloning Information for Gene/Insert 4

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer ovM2T3D-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7-M2-S2-S3-S4T3D was a gift from Terence Dermody (Addgene plasmid # 33302)
  • For your References section:

    An improved reverse genetics system for mammalian orthoreoviruses. Kobayashi T, Ooms LS, Ikizler M, Chappell JD, Dermody TS. Virology. 2010 Mar 15;398(2):194-200. Epub 2009 Dec 29. 10.1016/j.virol.2009.11.037 PubMed 20042210