Addgene - pMSCV-Atoh1ER-IRES-GFP Plasmid Data

Plasmid 33335: pMSCV-Atoh1ER-IRES-GFP

Gene/insert name:
Atoh1
Insert size:
1200
Species:
M. musculus (mouse)
Entrez Gene:
Atoh1 (Hath1, MATH-1, Math1, bHLHa14)
Fusion protein or tag:
ER
Terminal:
C terminal on insert
Vector backbone:
pMSCV-IRES-GFP
(Search Vector Database)
Backbone manufacturer:
Addgene plasmid #33336
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
6575
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
pLXSN 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (3)
Principal Investigator:
Martine Roussel
Principal Investigator:
Charles Sherr
Terms and Licenses:
MTA

Comments:

ER = estrogen-responsive element engineered to be induced by 4-hydroxytamoxifen (4-HT)

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Atoh1 inhibits neuronal differentiation and collaborates with Gli1 to generate medulloblastoma-initiating cells. Ayrault et al (Cancer Res. 2010 Jul 1;70(13):5618-27. Epub 2010 Jun 1. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 33335" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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