pMSCV-Atoh1ER-IRES-GFP
(Plasmid #33335)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pMSCV-IRES-GFP
  • Backbone manufacturer
    Addgene plasmid #33336
  • Backbone size w/o insert (bp) 6575
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Atoh1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1200
  • Entrez Gene
    Atoh1 (a.k.a. Hath1, MATH-1, Math1, bHLHa14)
  • Tag / Fusion Protein
    • ER (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pLXSN 5'
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

ER = estrogen-responsive element engineered to be induced by 4-hydroxytamoxifen (4-HT)

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-Atoh1ER-IRES-GFP was a gift from Martine Roussel & Charles Sherr (Addgene plasmid # 33335)
  • For your References section:

    Atoh1 inhibits neuronal differentiation and collaborates with Gli1 to generate medulloblastoma-initiating cells. Ayrault O, Zhao H, Zindy F, Qu C, Sherr CJ, Roussel MF. Cancer Res. 2010 Jul 1;70(13):5618-27. Epub 2010 Jun 1. 10.1158/0008-5472.CAN-09-3740 PubMed 20516124