Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pSfi-HspLacZ
(Plasmid #33351)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 33351 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2800
  • Vector type
    Mammalian Expression, Mouse Targeting

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Hsp68 promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4500
  • Entrez Gene
    Hspa1a (a.k.a. Hsp70-3, Hsp70.3, Hsp72, hsp68, hsp70A1)
  • Promoter Hsp68 (Hspa1a)
  • Tag / Fusion Protein
    • LacZ (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer LacZ-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

For more information about this plasmid see attached information sheet.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSfi-HspLacZ was a gift from David Kingsley & Doug Mortlock (Addgene plasmid # 33351 ; http://n2t.net/addgene:33351 ; RRID:Addgene_33351)
  • For your References section:

    A general approach for identifying distant regulatory elements applied to the Gdf6 gene. Mortlock DP, Guenther C, Kingsley DM. Genome Res. 2003 Sep;13(9):2069-81. Epub 2003 Aug 12. 10.1101/gr.1306003 PubMed 12915490