Addgene - pFRT-Tet 129 Plasmid Data

Plasmid 33359: pFRT-Tet 129

Gene/insert name:
TetR cassette
Insert size:
2400
Fusion protein or tag:
FRT site
Terminal:
N terminal on insert
Fusion protein or tag:
FRT site
Terminal:
C terminal on insert
Vector backbone:
pCRII
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mouse Targeting
Backbone size w/o insert (bp):
4100
Cloning site 5':
none
Site destroyed during cloning:
Unknown
Cloning site 3':
none
Site destroyed during cloning:
Unknown
5' sequencing primer:
M13 forward List of Sequencing Primers
3' sequencing primer:
M13 reverse
Bacterial resistance(s)
Ampicillin
Growth strain(s)
INValphaF'
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Supplemental document:
pFRT-Tet129_info_sheet (application/pdf)
Principal Investigator:
Doug Mortlock
Terms and Licenses:
MTA

Comments:

Do not select for this plasmid with tetracycline, as we have found that the Tet resistance cassette does not work reliably in high-copy plasmids such as this one. The Tet cassette MUST be transferred to a low-copy vector, e.g. a BAC, to allow Tet resistance in a reliable fashion. In BAC clones the Tet cassette has been extremely reliable.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 33359" in your Materials and Methods section.