Addgene - pIBG-FTet Plasmid Data

Plasmid 33361: pIBG-FTet

Gene/Insert 1
Gene/insert name:
IRES-Bgeo
Insert size:
5000
Gene/Insert 2
Gene/insert name:
TetR cassette
Insert size:
2400
Fusion protein or tag:
FRT site
Terminal:
N terminal on insert
Fusion protein or tag:
FRT site
Terminal:
C terminal on insert
Vector backbone:
pBluescript
(Search Vector Database)
Backbone manufacturer:
Stratagene
Vector type:
Mouse Targeting
Backbone size w/o insert (bp):
3100
Cloning information for Gene/Insert 1
Cloning site 5':
none
Site destroyed during cloning:
Unknown
Cloning site 3':
none
Site destroyed during cloning:
Unknown
5' sequencing primer:
none List of Sequencing Primers
3' sequencing primer:
LacZ-R
Cloning information for Gene/Insert 2
Cloning site 5':
none
Site destroyed during cloning:
Unknown
Cloning site 3':
none
Site destroyed during cloning:
Unknown
5' sequencing primer:
Neo-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH10B
Growth temperature (℃):
37
Growth instructions:
Do not select for this plasmid with tetracycline, as we have found that the Tet resistance cassette does not work reliably in high-copy plasmids such as this one. The Tet cassette MUST be transferred to a low-copy vector, e.g. a BAC, to allow Tet resistance in a reliable fashion. In BAC clones it has been extremely reliable.
High or low copy:
Unknown
Selectable markers:
Neomycin
Selectable markers, other:
tetracycline
Sequence: View sequences (5)
Map:
View map
Supplemental document:
pIBGFtet_info_sheet (application/pdf)
Supplemental document:
pIBGFtet_sequence (text/plain)
Principal Investigator:
Doug Mortlock
Terms and Licenses:
MTA

Comments:

Insert origin: IBG = Ires-Bgeo cassette from pGt1.8IresBgeo (Mountford et al (1994) PNAS 91:4303-4307); FRT-Tet-FRT cassette from pFRT129-NotX (Mortlock et al (2003) Genome Research 13:2069-2081).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Bmp2 transcription in osteoblast progenitors is regulated by a distant 3' enhancer located 156.3 kilobases from the promoter. Chandler et al (Mol Cell Biol. 2007 Apr;27(8):2934-51. Epub 2007 Feb 5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 33361" in your Materials and Methods section.