pBpuro_KLF4_ER
(Plasmid #34609)

• Depositing Lab: Michael Ruppert
• Publication: Foster et al Oncogene. 2005 Feb 24;24(9):1491-500.

Backbone

• Vector backbone: pBABE-puro
• Backbone manufacturer: Morganstern JP and Land H 1990
• Backbone size w/o insert (bp): 5169
• Modifications to backbone: none
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Growth instructions: use amp 100ug/ml
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KLF4
• Alt name: GKLF
• Alt name: EZF
• Species: H. sapiens (human)
• Insert Size (bp): 2400
• GenBank ID: AF105036
• Entrez Gene: KLF4 (a.k.a. EZF, GKLF)
• Promoter: LTR
• Tags / Fusion Proteins:
  • Myc epitope tag (N terminal on insert)
  • estrogen receptor fusion (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: use KLF4 internal primers
• 3′ sequencing primer: use KLF4 internal primers

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To prepare vector, a BamHI fragment encoding c-MYC was released from pBABE-puro-cMYC-ER. See Littlewood TD, Hancock DC, Danielian PS, Parker MG and
Evan GI. (1995). Nucleic Acids Res., 23, 1686–1690. A BamHI fragment encoding KLF4 was inserted into the same sites of this vector.