Addgene - pLJM60 Plasmid Data

Gene/insert name:
deptor
Species:
H. sapiens (human)
Entrez Gene:
DEPTOR (DEP.6, DEPDC6)
Fusion protein or tag:
Flag
Terminal:
N terminal on backbone
Vector backbone:
pLJM1-Flag
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert:
7400
Modifications to Backbone:
Made by cloning Age1- FLAG-Sal-gene-Not1-ExcoR1 into the Age1/Ecor1 sites in pLJM1
Promoter:
CMV
Cloning site 5':
SalI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
need custom
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
David Sabatini
Terms and Licenses:
MTA

Comments:

If you plan on using this plasmid for the vector, know that it has a CMV promoter, a FLAG tag and then genes cloned into Sal/Not sites in the vector. Region of interest: Age(site destroyed)-FLAG-Sal-Gene-Not-EcoR1

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 34610" in your Materials and Methods section.