LAMP1-mRFP-FLAG
(Plasmid #34611)

• Purpose: 3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tag
• Depositing Lab: David Sabatini
• Publication: Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

Backbone

• Vector backbone: pLJM1
• Backbone size w/o insert (bp): 7400
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LAMP1
• Species: R. norvegicus (rat)
• Insert Size (bp): 1253
• GenBank ID: NM_012857.2
• Entrez Gene: Lamp1 (a.k.a. LGP120)
• Tags / Fusion Proteins:
  • mRFP (C terminal on insert)
  • 2xFlag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: pLJM1-3 (5'-GACGTGAAGAATGTGCGAGA-3')

Resource Information

• Articles Citing this Plasmid:
  • 21 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry