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pRK5-TGF beta type I receptor (mL45TD)
(Plasmid #34702)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 34702 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRK5 modified with flag
  • Backbone manufacturer
    Genentech
  • Backbone size w/o insert (bp) 4700
  • Modifications to backbone
    Flag tag with stop codon at the end is inserted between SalI and HindIII.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TGF beta type I receptor
  • Alt name
    TGFb type I receptor
  • Alt name
    Tgfbr1
  • Alt name
    R4mL45TD
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1506
  • Mutation
    T202D, N265A, D267A, N268A
  • Entrez Gene
    Tgfbr1 (a.k.a. Alk5, Skr4, Tgfr-1, tbetaR-I)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This plasmid was derived from pRK5-TbRI(T202D)-FLAG, Addgene plasmid #14833.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

3 amino acids, GSM, were introduced after aa148 for cloning purposes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-TGF beta type I receptor (mL45TD) was a gift from Ying Zhang (Addgene plasmid # 34702 ; http://n2t.net/addgene:34702 ; RRID:Addgene_34702)
  • For your References section:

    TGF-beta receptor-activated p38 MAP kinase mediates Smad-independent TGF-beta responses. Yu L, Hebert MC, Zhang YE. EMBO J. 2002 Jul 15;21(14):3749-59. 10.1093/emboj/cdf366 PubMed 12110587