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pAD12
(Plasmid #34832)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 34832 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2961
  • Modifications to backbone
    T7 oligo was ligated into SacI/SpeI sites in pBluescript (SK+), thereby removing the XbaI, Not I, Sac II, BstX I sites.
  • Vector type
    Bacterial Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer M13F(-20)
  • 3′ sequencing primer M13R
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Andrew Dillin & Cynthia Kenyon, UCSF.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use HT115 bacteria for experiments--Grow O/N on LB supplemented with carbenicillin (100ug/ml) & tetracyclin (12.5ug/ml) and pick single colony for liquid culture.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAD12 was a gift from Cynthia Kenyon (Addgene plasmid # 34832 ; http://n2t.net/addgene:34832 ; RRID:Addgene_34832)
  • For your References section:

    Timing requirements for insulin/IGF-1 signaling in C. elegans. Dillin A, Crawford DK, Kenyon C. Science. 2002 Oct 25;298(5594):830-4. 10.1126/science.1074240 PubMed 12399591