Addgene - pTY1048-S65T-LacI Plasmid Data

Plasmid 34853: pTY1048-S65T-LacI

Gene/insert name:
GFP-LacI
Mutation:
GFP is mutated: S65T and contains introns, in order to increase expression of the fusion protein
Vector backbone:
pTY1048
(Search Vector Database)
Vector type:
Worm Expression
Cloning site 5':
None
Site destroyed during cloning:
Unknown
Cloning site 3':
None
Site destroyed during cloning:
Unknown
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
lacI-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Paul Sternberg
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Visualization of C. elegans transgenic arrays by GFP. Gonzalez-Serricchio et al (BMC Genet. 2006 Jun 7;7:36. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 34853" in your Materials and Methods section.