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pCFJ594 - pENTR[1-2](NeoR)
(Plasmid #34878)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 34878 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDONRP2-P3R
  • Vector type
    Worm targeting
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NeoR
  • Promoter rps-27

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We would like to thank Rosina Giordano-Santini and Denis Dupuy for sending us the original Neomycin Resistance plasmid. For details on how to use the neomycin selection in C. elegans and other nematode species please see: Giordano-Santini R, Milstein S, Svrzikapa N, Tu D, Johnsen R, Baillie D, Vidal M, Dupuy D. Nat Methods. 2010 Sep;7(9):721-3. An antibiotic selection marker for nematode transgenesis.
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCFJ594 - pENTR[1-2](NeoR) was a gift from Erik Jorgensen (Addgene plasmid # 34878 ; http://n2t.net/addgene:34878 ; RRID:Addgene_34878)
  • For your References section:

    Improved Mos1-mediated transgenesis in C. elegans. Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM. Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865. 10.1038/nmeth.1865 PubMed 22290181