Addgene - p6604 MSCV-IP N-HAonly PP2CA Plasmid Data

Plasmid 35005: p6604 MSCV-IP N-HAonly PP2CA

Gene/insert name:
PP2CA
Species:
H. sapiens (human)
Entrez Gene:
PP2CA (AT3G11410, AHG3, ARABIDOPSIS THALIANA PROTEIN PHOSPHATASE 2CA, ATPP2CA, protein phosphatase 2CA)
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Vector backbone:
MSCV-IP N-HAonly
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
8100
Modifications to Backbone:
NTap mod. by E.White to remove Flag
Promoter:
MSCV LTR
Cloning method:
Gateway Cloning
5' sequencing primer:
5' CTACATCGTGACCTGGGAAGC 3' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Peter Howley
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Systematic identification of interactions between host cell proteins and E7 oncoproteins from diverse human papillomaviruses. White et al (Proc Natl Acad Sci U S A. 2012 Jan 9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35005" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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