Addgene - p6640 MSCV-P C-FlagHA 16E7-Kozak Plasmid Data

Plasmid 35018: p6640 MSCV-P C-FlagHA 16E7-Kozak

Gene/insert name:
HPV16 E7
Insert size:
300
Species:
HPV 16
Entrez Gene:
E7 (HpV16gp2)
Fusion protein or tag:
HA
Terminal:
C terminal on backbone
Fusion protein or tag:
FLAG
Terminal:
C terminal on backbone
Vector backbone:
MSCV-P C-FlagHA
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
8700
Promoter:
MSCV LTR
Cloning method:
Gateway Cloning
5' sequencing primer:
5' CTACATCGTGACCTGGGAAGC 3' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (2)
Principal Investigator:
Peter Howley
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Systematic identification of interactions between host cell proteins and E7 oncoproteins from diverse human papillomaviruses. White et al (Proc Natl Acad Sci U S A. 2012 Jan 9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35018" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with

13733: cmv 16 E7 Flag/HA

8643: p1324 HPV-16 E7

8641: p1321 HPV-16 E6/E7