Addgene - p53-(1-393)-E285A-N268D Plasmid Data

Plasmid 35090: p53-(1-393)-E285A-N268D

Gene/insert name:
p53
Insert size:
1179
Species:
H. sapiens (human)
Entrez Gene:
TP53 (BCC7, LFS1, P53, TRP53)
Fusion protein or tag:
His
Terminal:
N terminal on backbone
Mutation:
Contains aa 1-393 - E285A-N268D
Vector backbone:
pET15b
(Search Vector Database)
Backbone manufacturer:
Novagen
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
5700
Promoter:
T7
Cloning site 5':
NdeI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T7-term
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Cheryl Arrowsmith
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Comments:

Protein expression in BL21DE3-pLysS. Induce culture around OD600=0.8 with 0.5mM IPTG, 15 oC overnight.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Molecular basis of Pirh2-mediated p53 ubiquitylation. Sheng et al (Nat Struct Mol Biol. 2008 Dec . 15(12):1334-42. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35090" in your Materials and Methods section.