Addgene - pPGK-GZF1-RR Plasmid Data

Plasmid 35092: pPGK-GZF1-RR

Gene/insert name:
ZFN GZF1-Fok(RR)
Insert size:
1000
Species:
synthetic
Fusion protein or tag:
HA
Terminal:
N terminal on insert
Fusion protein or tag:
SV40 NLS
Terminal:
N terminal on insert
Mutation:
contains the "RR" obligate heterodimer variant
Vector backbone:
pPGK
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4500
Promoter:
PGK
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
AgeI
Site destroyed during cloning:
No
5' sequencing primer:
HAtag-f: ATGTACCCATACGATGTCCCAGACTACG List of Sequencing Primers
3' sequencing primer:
FokLinkSeq-r: TCTATCCTGAGTGGAATTTCTGGC
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Map:
View map
Principal Investigator:
David Segal
Terms and Licenses:
MTA

Comments:

Originally described in Szczepek, M., Brondani, V., Büchel, J., Serrano, L., Segal, D.J. and Cathomen, T. (2007) Structure-based redesign of the dimerization interface reduces the toxicity of zinc finger nucleases. Nat. Biotechnol., 25:786-793.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The generation of zinc finger proteins by modular assembly. Bhakta et al (Methods Mol Biol. 2010;649:3-30. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35092" in your Materials and Methods section.