Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35155||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5597
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameMyc promoter
SpeciesH. sapiens (human)
Insert Size (bp)2500
MutationContains 2.5 kb of the c-myc minimal promoter
Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
- Promoter c-myc
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site SacI (not destroyed)
- 5′ sequencing primer EBV-rev
- 3′ sequencing primer Luc_N_Rev (Common Sequencing Primers)
This plasmid is a c-myc promoter-luciferase reporter construct designed to examine the regulation of transcription in response to conditional and constitutive Myc activation and functions as described in the associated publication.
Addgene's sequencing results identified a V13C mutation in exon 1 of c-myc when compared to GenBank reference sequence AAA59886.1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HBM-Luc was a gift from Linda Penn (Addgene plasmid # 35155 ; http://n2t.net/addgene:35155 ; RRID:Addgene_35155)
For your References section:The Myc negative autoregulation mechanism requires Myc-Max association and involves the c-myc P2 minimal promoter. Facchini LM, Chen S, Marhin WW, Lear JN, Penn LZ. Mol Cell Biol. 1997 Jan;17(1):100-14. PubMed 8972190