Addgene - pLKO-tet-puro-sh-control Plasmid Data

Plasmid 35163: pLKO-tet-puro-sh-control

Gene/insert name:
shRNAi against GFP#1
Insert size:
55
shRNA sequence:
GCAAGCTGACCCTGAAGTTCAT
Vector backbone:
pLKO-tet-puro
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size w/o insert (bp):
10000
Promoter:
hPGK
Cloning site 5':
AgeI
Site destroyed during cloning:
Unknown
Cloning site 3':
EcoRI
Site destroyed during cloning:
Unknown
5' sequencing primer:
H1 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Principal Investigator:
Benjamin Blencowe
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: An alternative splicing switch regulates embryonic stem cell pluripotency and reprogramming. Gabut et al (Cell. 2011 Sep 30;147(1):132-46. Epub 2011 Sep 15. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35163" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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