Addgene - pLKO-tet-puro-sh-ex16b Plasmid Data

Plasmid 35168: pLKO-tet-puro-sh-ex16b

Gene/insert name:
Foxp1
shRNA sequence:
GACGAGCTTGGCGCACATAAC
Species:
M. musculus (mouse)
Entrez Gene:
Foxp1 (3110052D19Rik, 4932443N09Rik, AI461938, AW494214)
Vector backbone:
pLKO.1
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size w/o insert (bp):
7000
Cloning site 5':
AgeI
Site destroyed during cloning:
Unknown
Cloning site 3':
EcoRI
Site destroyed during cloning:
Unknown
5' sequencing primer:
H1 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Benjamin Blencowe
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: An alternative splicing switch regulates embryonic stem cell pluripotency and reprogramming. Gabut et al (Cell. 2011 Sep 30;147(1):132-46. Epub 2011 Sep 15. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35168" in your Materials and Methods section.