Addgene - MSCV-Myc-IRES-GFP Plasmid Data

Plasmid 35396: MSCV-Myc-IRES-GFP

Gene/Insert 1
Gene/insert name:
Myc
Alt name:
C-Myc
Insert size:
1346
Species:
M. musculus (mouse)
GenBank ID:
NM_010849
Entrez Gene:
Myc (AU016757, Myc2, Niard, Nird, bHLHe39)
Gene/Insert 2
Gene/insert name:
GFP
Alt name:
green fluorescent protein
Species:
Aequorea Victoria
Vector backbone:
MSCV
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Cloning information for Gene/Insert 1
Cloning site 5':
BamHI
Site destroyed during cloning:
Unknown
Cloning site 3':
XhoI
Site destroyed during cloning:
Unknown
5' sequencing primer:
5’ GGATCCACGACGATGCCCCTCAACGTGAAC List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL1 Blue
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Martine Roussel
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A mouse model of the most aggressive subgroup of human medulloblastoma. Kawauchi et al (Cancer Cell. 2012 Feb 14;21(2):168-80. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35396" in your Materials and Methods section.