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pBY2727
(Plasmid #35399)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Modifications to backbone
    5' to the EGFP gene the coding sequences for 2 SB1 epitopes and 2 TEV cleavage sites was inserted 3' to the EGFP gene the AviTag encoding sequence was inserted
  • Vector type
    Mammalian Expression, Mouse Targeting
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Gene/Insert

  • Gene/Insert name
    SnAvi-tag
  • Species
    Synthetic
  • Insert Size (bp)
    921
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer pEGFP_C2-RP
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBY2727 was a gift from Ralf Baumeister (Addgene plasmid # 35399)
  • For your References section:

    SnAvi - a new tandem tag for high-affinity protein-complex purification. Schaffer U, Schlosser A, Muller KM, Schafer A, Katava N, Baumeister R, Schulze E. Nucleic Acids Res. 2010 Jan 4. ():. 10.1093/nar/gkp1178 PubMed 20047968