|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35399||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Modifications to backbone5' to the EGFP gene the coding sequences for 2 SB1 epitopes and 2 TEV cleavage sites was inserted 3' to the EGFP gene the AviTag encoding sequence was inserted
Vector typeMammalian Expression, Mouse Targeting
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Insert Size (bp)921
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer pEGFP_C2-RP (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBY2727 was a gift from Ralf Baumeister (Addgene plasmid # 35399)
For your References section:SnAvi - a new tandem tag for high-affinity protein-complex purification. Schaffer U, Schlosser A, Muller KM, Schafer A, Katava N, Baumeister R, Schulze E. Nucleic Acids Res. 2010 Jan 4. ():. 10.1093/nar/gkp1178 PubMed 20047968
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.