pBY2727
(Plasmid #35399)

• Depositing Lab: Ralf Baumeister
• Publication: Schaffer et al Nucleic Acids Res. 2010 Jan 4. ():.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Modifications to backbone: 5' to the EGFP gene the coding sequences for 2 SB1 epitopes and 2 TEV cleavage sites was inserted 3' to the EGFP gene the AviTag encoding sequence was inserted
• Vector type: Mammalian Expression, Mouse Targeting
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SnAvi-tag
• Species: Synthetic
• Insert Size (bp): 921
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: pEGFP_C2-RP

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry