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pRSII423
(Plasmid #35464)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 35464 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS42x
  • Backbone manufacturer
    Stratagene
  • Backbone size (bp) 5801
  • Modifications to backbone
    2 micron replication origin is from YEplac195 and has had its XbaI site removed.
  • Vector type
    Yeast 2 micron vector
  • Promoter None
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer M13 Forward, T7
  • 3′ sequencing primer M13 Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Daniel J Lew, Duke University Medical Center Chandra Tucker, Duke University (now at University of Colorado, Denver)
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HIS3 has been modified to remove HindIII, KpnI and PstI sites without altering the amino acid sequence of the His3 protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSII423 was a gift from Steven Haase (Addgene plasmid # 35464 ; http://n2t.net/addgene:35464 ; RRID:Addgene_35464)
  • For your References section:

    New and Redesigned pRS Plasmid Shuttle Vectors for Genetic Manipulation of Saccharomycescerevisiae. Chee MK, Haase SB. G3 (Bethesda). 2012 May;2(5):515-26. Epub 2012 May 1. 10.1534/g3.111.001917 PubMed 22670222