Addgene - pAAV-Ef1a-DIO hChR2(E123A)-EYFP Plasmid Data

Plasmid 35507: pAAV-Ef1a-DIO hChR2(E123A)-EYFP

Gene/insert name:
hChR2
Insert size:
1661
Species:
Chlamydomonas reinhardtii
Fusion protein or tag:
EYFP
Terminal:
C terminal on insert
Mutation:
E123A
Vector backbone:
pAAV
(Search Vector Database)
Backbone manufacturer:
Stratagene
Vector type:
Mammalian Expression ; AAV
Backbone size w/o insert (bp):
5605
Modifications to Backbone:
Addition of an Ef1a promoter, lox sites and WPRE
Promoter:
Ef1a
Cloning site 5':
AscI
Site destroyed during cloning:
No
Cloning site 3':
NheI
Site destroyed during cloning:
No
5' sequencing primer:
CACCCACACAAAGGAAAAGGGCC List of Sequencing Primers
3' sequencing primer:
GCAATAGCATGATACAAAGG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Karl Deisseroth
Terms and Licenses:
MTA

Comments:

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Principles for applying optogenetic tools derived from direct comparative analysis of microbial opsins. Mattis et al (Nat Methods. 2011 Dec 18;9(2):159-72. doi: 10.1038/nmeth.1808. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35507" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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